- Catalog number:
- E-CK-A151
- Applications:
- Apoptosis
- Size:
- 10mL
- Transportation temperature:
- 2~8°C
- Storage:
- 2~8°C
- Leadtime:
- 7~10 days
- Producer manual
Introduction
Elabscience® Annexin V Binding Buffer (10 ×) is developed to identify apoptotic and necrotic cells.
Components
Cat. | Products | 10 mL | 50 mL | Storage |
E-CK-A151 | Annexin V Binding Buffer(10 ×) | 10 mL | 50 mL | 2~8°C |
Manual | One Copy | |||
Self-Prepared Reagent
DI water
Instructions
The Annexin V Binding Buffer (10 ×)[E-CK-A151] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use.
For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.
Staining Procedure
1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to the cells and resuspend the cells gently followed by the cell counting.
Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware！
2. Split the cell suspension into tubes, 1~5 × 105 cells for each, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer to resuspend the cells.
3. Add 5 µl of Annexin V- fluorescein and 5 µl of DNA dye (PI[E-CK-A161] or 7-AAD[E-CK-A162]or DAPI[E-CK-A163]) to each tube.
4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.
5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.
Storage
Store at 2~8°C for one year.
Introduction
Elabscience® Annexin V Binding Buffer (10 ×) is developed to identify apoptotic and necrotic cells.
Components
Cat. | Products | 10 mL | 50 mL | Storage |
E-CK-A151 | Annexin V Binding Buffer(10 ×) | 10 mL | 50 mL | 2~8°C |
Manual | One Copy | |||
Self-Prepared Reagent
DI water
Instructions
The Annexin V Binding Buffer (10 ×)[E-CK-A151] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use.
For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.
Staining Procedure
1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to the cells and resuspend the cells gently followed by the cell counting.
Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware！
2. Split the cell suspension into tubes, 1~5 × 105 cells for each, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer to resuspend the cells.
3. Add 5 µl of Annexin V- fluorescein and 5 µl of DNA dye (PI[E-CK-A161] or 7-AAD[E-CK-A162]or DAPI[E-CK-A163]) to each tube.
4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.
5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.
Storage
Store at 2~8°C for one year.
Introduction
Elabscience® Annexin V Binding Buffer (10 ×) is developed to identify apoptotic and necrotic cells.
Components
Cat. | Products | 10 mL | 50 mL | Storage |
E-CK-A151 | Annexin V Binding Buffer(10 ×) | 10 mL | 50 mL | 2~8°C |
Manual | One Copy | |||
Self-Prepared Reagent
DI water
Instructions
The Annexin V Binding Buffer (10 ×)[E-CK-A151] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use.
For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.
Staining Procedure
1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to the cells and resuspend the cells gently followed by the cell counting.
Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware！
2. Split the cell suspension into tubes, 1~5 × 105 cells for each, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer to resuspend the cells.
3. Add 5 µl of Annexin V- fluorescein and 5 µl of DNA dye (PI[E-CK-A161] or 7-AAD[E-CK-A162]or DAPI[E-CK-A163]) to each tube.
4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.
5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.
Storage
Store at 2~8°C for one year.
Introduction
Elabscience® Annexin V Binding Buffer (10 ×) is developed to identify apoptotic and necrotic cells.
Components
Cat. | Products | 10 mL | 50 mL | Storage |
E-CK-A151 | Annexin V Binding Buffer(10 ×) | 10 mL | 50 mL | 2~8°C |
Manual | One Copy | |||
Self-Prepared Reagent
DI water
Instructions
The Annexin V Binding Buffer (10 ×)[E-CK-A151] is a 10 × concentrated solution. Dilute with DI water to 1 × working solution before use.
For example: Take 1 mL Annexin V Binding Buffer (10 ×), dilute with DI water to 10 mL.
Staining Procedure
1. Induce apoptosis of suspension cells with reagents of interest. Collect cell cultures, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to the cells and resuspend the cells gently followed by the cell counting.
Note: This product is only validated in suspension cells. Good cell viability is the key to the experiment. When the adherent cells are used for apoptotic detection, treatments like digestion may increase the ratio of necrotic or apoptotic cells and cause uncontrollable effects on the experimental results. Please be aware！
2. Split the cell suspension into tubes, 1~5 × 105 cells for each, centrifuge at 300 g for 5 min and discard the supernatant. Add PBS to wash the cells and discard the supernatant. Add 500 μL of 1 × Annexin V Binding Buffer to resuspend the cells.
3. Add 5 µl of Annexin V- fluorescein and 5 µl of DNA dye (PI[E-CK-A161] or 7-AAD[E-CK-A162]or DAPI[E-CK-A163]) to each tube.
4. Gently vortex the cells and incubate at room temperature for 15-20 min in the dark.
5. Analyze the cells immediately with proper machine settings. Otherwise, place the cells on ice in the dark and analyze within 1 h.
Storage
Store at 2~8°C for one year.
