This ELISA kit applies to the in vitro quantitative determination of Human TnTf/TNNT3 concentrations in serum, plasma and other biological fluids.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with
an antibody specific to Human TnTf/TNNT3. Standards or samples are added to the micro ELISA plate wells and
combined with the specific antibody. Then a biotinylated detection antibody specific for Human TnTf/TNNT3 and
Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free
components are washed away. The substrate solution is added to each well. Only those wells that contain Human
TnTf/TNNT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzymesubstrate
reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of
Human TnTf/TNNT3. You can calculate the concentration of Human TnTf/TNNT3 in the samples by comparing the OD
of the samples to the standard curve.
