Intended use
This ELISA kit can be applied to the in vitro quantitative determination of Human HLA-B27 concentrations in serum, plasma and other biological fluids.
Test principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human HLA-B27. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human HLA-B27 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human HLA-B27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450nm ± 2 nm. The OD value is proportional to the concentration of Human HLA-B27. The concentration of Human HLA-B27 in samples can be calculated by comparing the OD of the samples with the standard curve.
