Intended use
This ELISA kit applies to the in vitro quantitative determination of Human β-EP concentrations in serum, plasma and other biological fluids.
Test principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated wit Human β EP. During the reaction, Human β-EP in the sample or standard competes with a fixed amount of Human β- EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-EP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human β-EP in the samples is then determined by comparing the OD of the samples to the standard curve.
