Helicobacter pylori has a worldwide distribution and a high prevalence. The infection with H. pylori is well established as a major cause of gastric and duodenal ulcers. The persistent infection with H. pylori is a risk factor for the development of gastric carcinoma and lymphoma. The infection produces elevated levels of specific H. pylori IgG and IgA antibodies in serum. IgM specific levels has not proven useful in the clinical laboratory. ELISA tests for the detection of H. pylori antibodies are sensitive, specific and cost effective in untreated patiens. The detection of H. pylori specific IgA alone is less sensitive than the detection of specific IgG antibodies. In untreated persons specific IgG and IgA remain elevated for years and successful eradication decreases the IgG and IgA levels, although in some individuals specific antibodies can persist during a long time. Because the infection with H. pylori is so prevalent the test should be performed only on individuals with symptoms. The prevalence of H. pylori antibodies increases with the age. A positive result only indicates that the patient has antibodies to H. pylori and if the individual has not been treated, a positive result very likely indicates an active infection with H. pylori. A definitive diagnosis should be given only when the clinical signs and symptoms of the patient are compatible. Detection methods based on chemiluminescence have received much attention due to their low background, linearity and wide dynamic range. When coupled to enzyme immunoassays, the signal amplification effect provided by theenzyme enables the design of CLIA (ChemiLuminescent ImmunoAssay) tests with shorter incubation times while keeping or improving their sensitivity.
Vircell
- це біотехнологічна компанія, що спеціалізується на розробці та виробництві готових до використання реагентів для інфекційних захворювань людини. Компанія присутня в лабораторіях понад 80 країн світу на п'яти континентах. Vircell пропонує понад 400 посилань на виявлення бактерій, вірусів, паразитів за допомогою різних методик: ІФА, хемілюмінесценція, пряма та непряма імунофлуоресценція, імунокаптаційна аглютинація, культура клітин та молекулярна біологія (олігохроматографія та контроль ПЛР).
Компанія Vircell, Гранада - Іспанія
